Peer-reviewed publication

From 546 colonies to 27 in 180 days

The system was clinically tested on a ward of University Hospital Bratislava, Ružinov. Over six months of daily application, the number of colonies isolated from the air fell by 95%. The study was carried out by the hospital department together with the Faculty of Medicine of Comenius University, and the results underwent peer review in the journal Antibiotics.

Day 0546 colonies

Study design

The microbial load of a room is not usually measured

In the study, it was measured for six months on a fully occupied internal medicine ward with patients, staff and visitors, an environment into which microorganisms are continually reintroduced.

  • Site

    UNB Ružinov, 5th Department of Internal Medicine

    University Hospital Bratislava together with the Department of Hospital Hygiene and Epidemiology and the Faculty of Medicine of Comenius University. The study's co-authors also include representatives of the manufacturer; the study contains a conflict of interest statement.

  • Duration

    Six months of measurement

    Air: baseline measurement and after 30, 90 and 180 days. Surfaces: seven samplings up to day 210.

  • Groups compared

    Three comparison groups

    Daily application, application twice a week, and control rooms without dry fog disinfection.

Number of colonies isolated from the air

Measured at baseline and after 30, 90 and 180 days of system use

015030045060003090180days of system use5464392082727543488320156156542570559565565
Show table
RegimeBaseline30 days90 days180 daysChange
Daily54643920827−95%
Twice a week543488320156−71.3%
Control (no disinfection)542570559565+4%

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Results

Results after 180 days

In the rooms where the system was applied, the microbial load of the air fell with each successive measurement. In the control rooms, it remained unchanged.

Air

The reduction was marked and persisted throughout the measurement period. Daily application achieved a greater reduction than application twice a week, but the difference between the regimes was not statistically significant: after 180 days, the daily regime reached 27 colonies, i.e. five per cent of the baseline value.

Surfaces

On surfaces, the reduction over time in the treated rooms was statistically significant (p = 0.010). Compared with the control rooms, however, it did not reach significance (p = 0.063 for daily application). Surfaces are continually recontaminated by touch, so a single percentage figure is not given.

95%

average reduction in airborne microbial load

daily
application:
95% reductionfrom 546 to 27 colonies
application
twice a week:
71.3% reductionfrom 543 to 156 colonies
combined result
of both regimes:
83.2% reductionfrom 1,089 to 183 colonies
statistically
significant reduction:
p < 0.001

The stated efficacy was achieved with daily application at a product concentration of 6% in two cycles of 6 minutes each, with the room kept closed for a further 45 minutes after application in accordance with the manufacturer's instructions. In the publication, the system is referred to as Hydrofogg™ by Detectair Technologies.

Conclusion

The study authors' conclusions

The study authors state that dry fog disinfection effectively reduces microbial load even in open areas with heavy foot traffic, and recommend it as part of a multi-component infection control strategy, i.e. as a supplement to routine cleaning and disinfection, not as a replacement for them.

Demonstrated results

  • (p < 0.001)

    Strong downward trend in airborne microbial load

  • (p = 0.002)

    Significant effect of daily application compared with control rooms

  • (p = 0.010)

    Reduction over time on surfaces in treated rooms

  • Reduction

    Reduction in several specific species of microorganisms

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Studies and reports

Library of studies and measurement reports

The published study is freely available. We will send you the measurement reports from individual facilities by email.

  • Public2024

    Efficacy of Antimicrobial Dry Fog in Improving the Environmental Microbial Burden in an Inpatient Ward

    Peer-reviewed study conducted at UNB Ružinov (5th Department of Internal Medicine, inpatient ward) in cooperation with the manufacturer. Six months of daily application, measurement of the microbial load of air and surfaces.

    Antibiotics 2024, 13, 1187doi: 10.3390/antibiotics13121187

  • Public2025

    Statistical evaluation of measurements: forensic pathology, St. Cyril and Methodius Hospital

    Cold storage room, large autopsy room and other rooms; sampling on 16 October 2025. Evaluation by the accredited laboratory ALS SK (SNAS 051/S-104).

    ALS SK

  • 2025

    Consolidated measurement report: AGEL Hospital Valašské Meziříčí

    Operating theatre, April to October 2025 (6 months). Evaluation by ALS SK.

    ALS SK

    Get the reportWe will send it to you by email
  • Public2025

    Summary measurement report: University Hospital Nitra

    Operating theatres No. 2 and 4 (theatre No. 1 as control), April to November 2025.

    Summary report

  • 2025

    Consolidated measurement report: UNB, Academician L. Dérer Hospital (ODCH)

    Long-term care department, 2 rooms, September to November 2025. Evaluation by ALS SK.

    ALS SK

    Get the reportWe will send it to you by email
  • 2024

    Result of a one-off dry fog disinfection test: University Hospital AGEL Skalica

    Super-aseptic operating theatre of the orthopaedic department, test on 27 November 2023, report dated 11 January 2024.

    University Hospital AGEL Skalica

    Get the reportWe will send it to you by email

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